Microfluidic polymerase chain reaction

A Microfluidic Platform for Screening and Selection of Monoclonal Antibodies from Single Cells

We report a microfluidic platform for rapid screening and selection of monoclonal antibodies (mAbs) secreted by single cells. The microfluidic platform integrates single-cell handling and compartmentalization, fluorescence detection of antibodies secreted by cells, and cell recovery for reverse transcription polymerase chain reaction (RT-PCR) of antibody genes.

Reduction of water evaporation in polymerase chain reaction microfluidic devices based on oscillating-flow.

Producing polymeric or hybrid microfluidic devices operating at high temperatures with reduced or no water evaporation is a challenge for many on-chip applications including polymerase chain reaction (PCR). We study sample evaporation in polymeric and hybrid devices, realized by glass microchannels for avoiding water diffusion toward the elastomer used for chip fabrication. The method dramatica...

DESIGN AND EXPERIMENTAL VALIDATION OF A MINIATURE REAL-TIME POLYMERASE CHAIN REACTION DEVICE USING DISPOSABLE MICROFLUIDIC CHIPS By

Microfluidic sonicator for real-time disruption of eukaryotic cells and bacterial spores for DNA analysis.

Biologic agent screening is a three-step process: lysis of host cell membranes or walls to release their DNA, polymerase chain reaction to amplify the genetic material and screening for distinguishing genetic signatures. Macrofluidic devices commonly use sonication as a lysis method. Here, we present a piezoelectric microfluidic minisonicator and test its performance. Eukaryotic human leukemia ...

Microfluidic Devices for Forensic DNA Analysis: A Review

Microfluidic devices may offer various advantages for forensic DNA analysis, such as reduced risk of contamination, shorter analysis time and direct application at the crime scene. Microfluidic chip technology has already proven to be functional and effective within medical applications, such as for point-of-care use. In the forensic field, one may expect microfluidic technology to become parti...

Polymerase chain reaction in microfluidic devices.

The invention of the polymerase chain reaction (PCR) has caused a revolution in molecular biology, giving access to a method of amplifying deoxyribonucleic acid (DNA) molecules across several orders of magnitude. Since the first application of PCR in a microfluidic device was developed in 1998, an increasing number of researchers have continued the development of microfluidic PCR systems. In th...